FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382752
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT68680.49669065747147717No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56180.40629122214250996No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT56050.40535106801508874No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT48500.3507498090763926No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44270.3201586401610701No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41820.30244035083659254No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.242270486681632No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29000.20972668996320382No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23430.16944470158061603No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA21210.1533897618661915No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC20180.1459408483950846No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA20130.14557925065376873No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.14333734465761033No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG18600.13451435976950313No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC16900.1222200365647636No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA16720.12091828469602647No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA15670.11332473212839324No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC14980.10833468329823424No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT14690.10623741639860221No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG14460.10457406678854922No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14440.10442942769202286No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.10247679988891717No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC14170.10247679988891717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT42750.057.2648621
TGGTATC44600.055.937762
CGTATCA705.638867E-1154.644481
CGTTAAC952.3646862E-1144.7365579
AAGTACG1300.042.5012661
GACGTTA1004.0017767E-1142.499737
GTATCAA173600.040.5671651
ATACGTG2400.037.188615
CGTTGTG1053.0504452E-936.4296572
CTTAACG701.8189894E-1236.42965320-21
TATACGT2750.035.5465134
ACGTTAA1202.8740033E-1035.416448
TGCTACG1150.035.10847518-19
GGTATCA86900.035.0183071
CGACTTT15250.034.83713
TATCAAC210850.034.7508542
TCGACTT15350.034.333272
CTCACGA751.4836003E-534.001013
GCATCGT1650.033.4846348
CAGTACG1404.1836756E-1133.393851
Produced by FastQC (version 0.11.5)