FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382752
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265691.921458077804263No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA16660.12048436740644743No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16590.11997813056860522No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15760.11397560806276179No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA14800.10703293142949712No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA14500.10486334498160191No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC14440.10442942769202286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG750.047.66569519
GCTACGC750.047.66569520
CGTTGTG303.4882753E-545.8406872
GTGGTAT48950.045.0829731
GGTCTCA257.8340666E-444.0261651
TGGTATC50850.043.48782
CACTGCT4400.041.8741516
CTTAACG753.6379788E-1240.3325121
CTGCTTA3700.040.13431518
GGCTCGG300.001920145136.6884731
CACGACG458.846728E-636.665925
ACTGCTT4400.034.99928717
TGCTTAA4450.034.60603719
ACGTTCT501.8200652E-533.0005239
GACGTTC501.8200652E-533.0005238
TAACGCA957.094059E-1131.84145723
GTCATCC1057.2759576E-1231.42906813
TGGTCGT1500.031.16603351
TACCACT6450.030.6981613
GCGTTGT453.782738E-430.5737291
Produced by FastQC (version 0.11.5)