FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973214
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68600.34765615893663837No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT66620.3376217683434235No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT59870.3034136185938271No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52630.26672221056611195No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT47000.23819007973793008No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38580.19551858034658176No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36880.18690319448372048No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27040.13703531395986446No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT22430.1136724146493994No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20620.10449956264247061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT43250.052.481361
TGGTATC44100.051.9451562
GTATCAA174100.042.115331
CGACTTT13900.036.996913
ATACGTG3800.036.90845
GGTATCA86600.036.6159971
TATCAAC207650.036.1249852
TCGACTT14750.034.5767482
TAGCGGT500.002788587734.002197
TATACGT4300.032.6167264
CGTGCAA3600.031.8770528
CAACGCA245950.030.8105475
ATCAACG247750.030.6210063
AACGCAG247850.030.5759056
CTGTGCG2550.030.0019349
TCAACGC254650.029.8413684
GTCGACT17100.029.8287491
GCGGTAT1302.4463588E-829.427211
CTAGTAC3350.027.910833
GCTACGC1800.027.15246220-21
Produced by FastQC (version 0.11.5)