FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424738
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT45950.32251543792613097No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32200.2260064657501941No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.22165478845935183No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.1752602934715014No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT21300.14950117144345135No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20000.14037668680136278No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.13055031872526737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1850.062.028773
TGGTATC23700.052.364342
GTGGTAT23950.052.00251
GCGTCAG2750.043.273944
GTATCAA88000.041.396181
CGTCAGA3000.039.667785
TATCTCG450.001662950837.778845
TATCAAC103550.036.0777852
TCGACTT9650.033.912872
CAGCGTC3600.033.0564842
GGTATCA43550.032.600211
CGACTTT10250.031.9277233
CAACGCA122250.030.6982025
ATCAACG123450.030.6407953
TCAACGC126650.029.7659344
GCAGCGT4150.029.7037981
GTCGACT11200.029.2236731
AACGCAG129650.029.1427366
TAGTACG600.006800526828.3381061
TTAAACG1850.025.27009422-23
Produced by FastQC (version 0.11.5)