FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424738
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217071.523578370198591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC150.00578440554.99877548
GTGGTAT23300.042.395591
GCTACGC403.9401984E-641.25052620
TGGTATC24100.040.859752
TACGCAG558.7010085E-735.00044622
GCCGACC350.004099705731.42897255
GTATCAA106050.029.8898681
CGGGAGC606.319772E-527.49938817
CTATCGG400.00787461527.49938612
AAGGTCG400.00787461527.49938611
CATACCG400.00787461527.49938639
ACCGTAT400.00787461527.49938638
TATCGGT400.00787461527.49938613
TATCAAC123700.025.7050132
AGAGTAC64350.025.38404811
CAGAGTA65450.025.04145810
GGTATCA47000.024.061691
TGCTACG701.8007627E-423.5717319
ACAACGC952.3170833E-623.1573774
CGAGCCT600.002036031822.91615729
Produced by FastQC (version 0.11.5)