FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555988
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT42360.27223860338254535No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.21683971855824083No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT31620.20321493481954872No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.17101674305971512No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT22320.14344583634321087No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.12493669616989334No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA17050.10957668053995274No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15950.10250721727931064No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15640.10051491399676604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24750.043.9661451
TGGTATC25300.042.500872
GTATCAA90400.041.3778461
TATCAAC107250.035.031022
GGTATCA44000.033.522041
ATCAACG118950.031.8354533
CGACTTT10150.031.823313
TCAACGC122650.030.8057654
CAACGCA123650.030.7294775
AGCGTCA2400.030.1047823
AACGCAG131850.028.8515136
TAGTACG905.1510473E-528.3375551
TATACGT3050.027.8694214
TACGTGC2350.027.1299576
CGCAGAG145850.025.9946618
GTCGACT12850.025.139931
ACGCAGA152150.025.0571827
TCGAGGG30600.024.3076139
GCAGAGT156800.024.287779
AGAGTAC71700.023.38587210-11
Produced by FastQC (version 0.11.5)