FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555988
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200161.2863852420455684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG358.671853E-539.28362712
TGGTATC29300.039.051082
GTGGTAT30050.038.6467251
ACGGGTG1101.2732926E-1129.99840416
GTATCAA104050.028.1148571
CGGTATC705.7010147E-627.5047252
CTCGGTA400.00787478427.4994226
TATCAAC120750.024.4182742
AGAGTAC62550.024.22333111
GGTATCA49200.023.9789243
CAGAGTA67000.023.31219510
TACGGGT4500.023.22098715
AGTACGT852.572134E-522.64585513
ACGGGCG1354.256435E-922.40621816
ATCAACG133550.021.8066833
TACATCG1901.8189894E-1221.70937241
TCAACGC135000.021.3891264
CAACGCA137250.021.0785585
AGTACGG68850.020.96838613
GAGTACG70200.020.80017712
Produced by FastQC (version 0.11.5)