FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980470
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT42150.4298958662682183No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.22672799779697492No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.20490173080257426No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.17287627362387428No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16540.1686946056483115No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT13880.14156475975807523No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.12126837129131945No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10480.10688751313145735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14200.050.2936361
GTCGACA901.2732926E-1147.2334441
TGGTATC15700.046.2937242
TATACGT701.8974242E-742.5035974
CGACTTT7550.041.6591573
GTATCAA58900.041.4996721
GATAGTC450.001662236637.780977
TCGACTT8550.037.2838552
TCGACAT1151.8189894E-1036.9596522
TATCAAC70950.034.7457162
GTCGACT9450.034.1880151
GGCTACG500.00278554134.008081
GGTATCA27650.033.8235861
TACGTGC652.550286E-432.6984066
ATCAACG77200.032.0428663
CGTCAGA1605.456968E-1231.8825741
TCAACGC78850.031.3723414
CAACGCA79850.031.085915
AACGCAG82150.030.2186666
AGCGTCA1003.0917563E-629.7525163
Produced by FastQC (version 0.11.5)