FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87170
Sequences flagged as poor quality0
Sequence length91
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT21572.474475163473672No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.46919811861879085No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.4141333027417689No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT3440.3946311804519904TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGG2890.3315360789262361No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.31662269129287596No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.29482620167488816No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.2925318343466789No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGAGCTA2070.23746701846965698No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1990.22828954915682004No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.2271423654927154No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC1920.22025926350808767TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGGCGT1780.2041986922106229No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.19387403923368132No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT1670.19157967190547207No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1600.1835493862567397No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1590.18240220259263507No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT1570.18010783526442584RNA PCR Primer, Index 11 (96% over 25bp)
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1400.16060571297464724No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.14798669266949638No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCGT1270.14569232534128715Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAGCTCGTATGCCGT1180.13536767236434552No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1150.13192612137203166No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATGTCGTATGCCGT1140.13077893770792703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC1120.12848457037971778No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA1080.1238958357232993No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG1040.1193071010668808No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.11586555007456693No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTTTGCCGT990.1135711827463577No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT950.1089824480899392No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.10668808076172995No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.1043937134335207No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT900.10324652976941608No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.10209934610531145No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.10095216244120685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)