FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87170
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1111312.748652059194676No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.15142824366181026No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC1200.13766203969255478No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC890.10209934610531145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT150.005766709354.99770417
CGAGGAT150.005766709354.99770416
TGCTTAA150.005766709354.99770419
CTGCTTA150.005766709354.99770418
GCACTGA150.005766709354.99770433
GCCGAGT150.005766709354.99770420
TACCACT257.818058E-443.99816513
GTGGTAT1900.039.1672021
TGGTATC1900.039.0773162
TCAACTC300.001916279336.665144
AGACAGG300.001916279336.665148
TGTAATG300.001916279336.665147
GTATCAA12750.033.7230451
AAGACGA501.8013783E-532.9986235
AGAAGGC350.004079899331.42725842
CAACTCA350.004079899331.4272585
TACGGGC701.5807018E-731.42725815
GCAATTA350.004079899331.42725821
CGGGCCG350.004079899331.42725817
ACGGGCC350.004079899331.42725816
Produced by FastQC (version 0.11.5)