FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168162
Sequences flagged as poor quality0
Sequence length91
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT28081.6698183894102117No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9190.5464968304373163No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.4798943875548578No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.4329158787359808No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT6230.37047608853367586No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.35382547781306123No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5900.3508521544700943No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.30446831031981064No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.2675991008670211No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT4140.24619117279765942TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4040.2402445261117256No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG3170.18850869994410152No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.18018339458379418No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.17602074190364053No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.14331418513100463No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2220.1320155564277304No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT2090.12428491573601647RNA PCR Primer, Index 11 (95% over 23bp)
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.1201222630558628No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2010.11952759838726942No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.11833826905008266No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.11774360438148927No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11477028103852238No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1870.11120229302696209No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGT1710.10168765832946801No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.10109299366087464No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA1690.10049832899228127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)