FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304945
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT92590.4017015590393697No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.19913707268503153No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.17583933673037752No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.15605578441134169No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33200.14403814407719057No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT28230.12247580744876777No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.10885292273785274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC31650.050.3562
GTGGTAT31650.049.9618231
GTCGACA1250.044.2081571
GCGTCAG3950.043.038454
GTATCAA128350.042.0276221
GCAGCGT4350.038.1104771
AGCGTCA4400.037.6708683
TATCAAC150850.036.795232
GGTATCA63050.036.271561
TCGACTT13950.033.5129132
CGACTTT14350.032.8749283
CAGCGTC5300.032.0758252
CGTCAGA5400.031.4831945
GTCGACT15750.031.3073641
CAACGCA182100.030.6688825
ATCAACG183200.030.6458033
CAGTACG1401.5006663E-930.3627451
AACGCAG187500.029.8996076
TCAACGC190550.029.4414164
ACGTGCA1703.965397E-1027.5014957
Produced by FastQC (version 0.11.5)