FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433018
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189351.3213372058131858No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA25060.1748756819523551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26750.039.0962521
TGGTATC27700.037.7369582
GTCGTTC300.001926243536.66487539
GCGCTAG300.001926243536.66487511
GCCTACG501.8200462E-533.0006946
TGGTCGT653.2076423E-629.6139451
GTATCAA108250.028.9835951
AGGTCGT705.70884E-627.49961722
CTGGTCG705.710388E-627.49865750
CGTAGGA400.00787564727.49865751
GTCGTTG507.0347806E-427.49865518
AGAGTAC62750.026.03060211
CGGGGTA6450.025.1538117
CAGAGTA65750.025.05200810
TGGGACG550.00122705224.998785
TATCAAC125850.024.9399452
GAGTACG69350.023.23606712
GGACGTC600.002035525922.91714747
AGACGAT600.00203635222.9155486
ATCAACG135500.022.811493
Produced by FastQC (version 0.11.5)