FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002628
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT97140.323516599458874No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.12595632892253053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86050.038.5813871
GTGGTAT23000.036.0399741
GGTATCA40850.034.9643021
TGGTATC23350.034.40232
TATCAAC100400.033.8663862
CGACTTT10000.033.5768283
AGCGTCA5800.032.2431373
CAACGCA106600.031.857865
ATCAACG107450.031.723463
TCAACGC110150.031.061614
TCGACTT11900.028.2158222
AACGCAG123600.027.854856
GCGTCAG6800.027.50154
GTCGACT12600.027.3270131
CGTCAGA6900.027.1038275
CGCAGAG131250.025.8409778
TAGACGC1658.6711225E-925.7602656
TAAGACG16650.025.2716484
ACGCAGA143000.023.9554527
GCAGAGT143300.023.6680269
Produced by FastQC (version 0.11.5)