FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800610
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181562.267770824746131No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTC202.613324E-454.99925216
CCTACGC150.005783284554.9992535
ACTACGG351.5714741E-647.14221213
GACGTAC257.8555767E-443.99944
GGTCCGA257.8555767E-443.999419
TGACGTA257.8555767E-443.99943
TATACGG257.8555767E-443.999417
TGGTATC10400.040.99272
GTGGTAT10550.039.381791
CTACGGG358.666062E-539.28517514
GCATACG401.9011839E-434.37453536
AGACGTG350.004099172531.4281413
CGTAACC350.004099172531.4281447
ACGTACT350.004099172531.428145
GCGCTAA350.004099172531.4281432
TTCGCCC350.004099172531.4281429
CAGACGT350.004099172531.4281421
ACTTACC453.7948304E-430.555149
GACTACG553.4898585E-529.99959212
GTATCAA45550.028.9346391
Produced by FastQC (version 0.11.5)