FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396318
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106752.6935440731937486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT150.005782085554.9969728
GCAAACC150.005782085554.9969737
TACTACG150.005782085554.996977
CACCGGT150.005782085554.9969727
GGACAGT150.005782085554.996976
ATTGTGT257.852359E-443.99757827
ACGGGTA451.8011087E-742.7754216
CTGGTGA403.934818E-641.24772610
GTCCTAA458.787139E-636.6924251
GGTCCAC300.001924623436.6646516
TAAACGA300.001924623436.664654
CCTCACC300.001924623436.6646524
CCGCCTC300.001924623436.6646521
TCGCGGG300.001924623436.6646515
GTGGTAT5900.036.381471
TGGTATC6050.035.9161762
ATATAGC558.6845466E-734.99807422
CGGGTAA401.8999193E-434.37310817
CGGGATT401.8999193E-434.37310817
GGGATTC401.8999193E-434.37310818
Produced by FastQC (version 0.11.5)