FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121744
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT33492.7508542515442236No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT5530.454231830726771TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.3967341306347746No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.2989880404783809No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.29652385333158104No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.2940596661847812No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.2595610461295834No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT2870.23574057037718493RNA PCR Primer, Index 11 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2490.20452753318438693No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT2430.19959915889078725No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGT2320.1905638060191878No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.17249310027598896No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT1930.15852937311078985No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGG1910.15688658167958996No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.13963727165199105No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT1700.13963727165199105No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGCTCTCGTATGCCGT1610.13224471021159154No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1580.1297805230647917No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCGT1460.11992377447759231No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.11745958733079248No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG1390.1141740044683927No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1108884216059929No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGGCGT1290.10596004731239321No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGTACTA1280.10513865159679327No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.10513865159679327No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT1270.10431725588119334No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1230.10103167301879354No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1220.1002102773031936No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAC302.2472002E-456.666671
TCGACTT1800.049.5833322
CGTATAA950.049.2105261
GTGGTAT2550.048.3333321
CGACTTT1900.046.9736823
GACTTTT2050.043.5365874
ATATACA409.28654E-442.52
ACCACTG255.4392673E-542.514-15
TGGTATC2950.041.7796632
GTATCAA11600.041.400861
GTCGTCT750.039.66666448-49
GGTATCA5850.037.7777751
TCAGATG709.180432E-636.4285743
TCGTCTG950.035.78947450-51
TAAGACG2150.035.5813985
TATCAAC13750.035.5454562
GTTCGGC301.591842E-435.41666850-51
CCGCACC301.591842E-435.41666884-85
GTCGACT2550.035.01
CTTGTGC751.8189894E-1234.050-51
Produced by FastQC (version 0.11.5)