FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6516791
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79380.12180841767059891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC54000.041.4787332
GTGGTAT57050.039.7894171
GTATCAA177650.038.7403561
TATCAAC205950.033.7002452
ATCAACG214150.032.3304443
GGTATCA90200.032.2824131
CAACGCA216200.032.0246245
AGCGTCA17800.031.7570613
TCAACGC221500.031.238444
AACGCAG239000.029.2554426
CGTCAGA19550.028.480215
GCGTCAG20450.027.4340084
CGCAGAG270850.025.5629448
ACGCAGA278600.025.0044047
GCAGCGT22500.024.7497461
GCAGAGT300150.023.194999
CAGCGTC27550.020.3639012
TCGAGGG61250.020.1932189
AGAGTAC134600.019.3095310-11
TATACGT5850.018.8897464
Produced by FastQC (version 0.11.5)