FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6516791
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG319730.49062491032779787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT62050.037.1671181
TGGTATC61600.037.0157972
GTATCAA216300.027.6859251
TATCAAC247100.023.9097372
AGAGTAC123650.023.26256211
ATCAACG260500.022.4432373
CAACGCA264650.022.0487235
GGTATCA104850.021.9001963
TCAACGC266350.021.8255714
AACGCAG277450.021.1902586
CAGAGTA136550.020.96423310
AGTACGG135250.020.47444713
GAGTACG142250.019.7762212
TACGGGG85200.018.26796315
GTACGGG150400.018.11934314
ACGCAGA329100.017.8145967
CGCAGAG328350.017.6879238
GCAGAGT346550.016.78289
CGAGGGG27150.016.71221710
ACGGGGG34050.016.3133916
Produced by FastQC (version 0.11.5)