FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233307
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65310.2924362839502137No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.2553612199308022No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.233420662721247No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT39620.1774050768658317No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34790.15577795618784163No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30460.1363896678781735No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22730.10177731946391608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT37650.050.6908761
TGGTATC37800.050.3705022
GCGTCAG3550.046.691314
GTATCAA155850.042.9558371
TATCAAC186250.036.5786252
GGTATCA76950.035.1867561
TCGACTT14650.033.6519662
CGACTTT14850.032.626353
ATCAACG215900.031.8504773
CAACGCA216500.031.6072865
TCAACGC220750.031.0936434
AACGCAG229600.029.9176586
GTCGACT17350.029.643831
AGCGTCA5650.029.3363593
ACGCCGA600.00680238728.3372141
TCGACAT1054.526848E-628.3334082
CAGTACG1255.6976387E-727.2037261
GCAGCGT6100.027.1758521
GCGTTGT1106.492948E-627.049161
CGTCAGA6600.025.7593755
Produced by FastQC (version 0.11.5)