FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233307
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243381.0897740436043948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT51800.041.921281
TGGTATC52350.041.5664372
ACGTATT300.001926338836.66539812
TGCTACG803.965397E-1034.3753519
CGGGGAC1950.029.61436317
GTATCAA200000.028.9811531
ACCCGTG606.320918E-527.4996647
CGCCGGA759.76043E-625.66577714
AGAGTAC117150.025.60944211
GTCGCCG651.0896697E-425.38373814
CAGAGTA120600.025.12765310
GTCGTAT1101.1819793E-825.0204221
CATCGTA550.001227004624.99969554
ACGGGTA5500.024.99857516
TATCAAC232200.024.9978852
TTAAACG4000.024.06274422
CGTAGCG701.8012391E-423.57166923
CGACCGC1302.752131E-923.26842510
GGTATCA96300.023.2167473
GTGTCCG600.00203653722.91587439
Produced by FastQC (version 0.11.5)