FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881578
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58790.31245050696808746No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT54580.29007567052761035No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.23049801815284832No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT38730.20583786587640798No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.17070777825846178No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29670.15768679268146205No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19220.10214830317956523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT35750.055.5333141
TGGTATC38800.051.482962
GTATCAA142750.041.067751
TGTACCG851.9290383E-840.000785
GGTATCA70700.035.4167481
TATCAAC172550.034.9267852
CGACTTT14400.033.646493
AGCGTCA5800.032.9747773
TCGACTT15650.030.9590662
ATCAACG196350.030.8880443
CAACGCA200700.030.345635
TCAACGC202500.030.0129264
GTCGACT17650.029.866871
AACGCAG210150.029.0424966
CAGCGTC6500.028.7697892
GCGTCAG6800.028.7505594
TACCTCG2450.027.755647
TCGAGGG55350.026.3374569
TATACGT3900.026.1543524
GCAGCGT7850.025.9946751
Produced by FastQC (version 0.11.5)