FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881578
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193041.0259473697077666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT43100.042.3353081
TGGTATC45400.040.4201432
CGACGTA453.797103E-430.55466810
GTATCAA176650.028.4792821
TTAAACG4500.028.11253722
AGAGTAC105150.025.13241811
TATCAAC205100.024.50762
CAGAGTA109000.024.44653910
TTAATCG952.3184093E-623.15660937
GGTATCA82100.022.979243
AAGTCCG600.00203635222.9160029
TGTACCG600.002036666522.9153945
ATCAACG224350.022.2487323
TCGTCTA1003.6165566E-622.00053254
TCAACGC227100.021.8558964
TGCTTAA7300.021.84984419
CAACGCA228300.021.7169285
GAGTACG120500.021.67986912
AGTACGG120950.021.57647313
AACGCAG234350.021.2618876
Produced by FastQC (version 0.11.5)