FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3856912
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67260.17438821523540077No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT58250.15102755779753335No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA55120.14291225726695345No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51270.13293017833956286No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT40160.10412475057766421No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT42250.049.1963961
TGGTATC50150.042.4582022
GTATCAA148350.042.2052351
TATCAAC179600.035.3067132
ATCAACG199050.032.347843
CAACGCA199850.032.154975
GGTATCA75150.031.7876531
TCAACGC205150.031.3652784
AACGCAG228600.028.2972686
AGCGTCA8800.027.0458243
CGACGCA808.6138095E-426.5632065
CGCAGAG247150.025.8635238
CGTCAGA9250.025.7304135
GCGTCAG9250.025.730084
GTCGACT18050.024.7265341
ACGCAGA264000.024.4861287
GCAGAGT260750.024.4819559
CGACTTT17550.024.2168523
GCAGCGT10000.023.803411
TCGACTT18650.023.0163972
Produced by FastQC (version 0.11.5)