FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3856912
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243740.6319563422758933No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA48320.1252815724081856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT55550.039.532921
TGGTATC58850.036.9214252
GTATCAA191700.027.7491531
GCGCGTT759.759655E-625.66685331
AGAGTAC107200.024.29229511
TATCAAC224600.023.8303762
GGTATCA95350.022.95713
CAGAGTA113300.022.93587110
ATCAACG246400.021.606743
CAACGCA248500.021.3574735
GGCACGT4000.021.31237819
TCAACGC250500.021.1869544
GAGTACG122650.020.94077112
GGTACCG804.4458362E-420.6243485
AGTACGG124600.020.61304713
ACGGGTA3350.020.52147316
AACGCAG262350.020.2718966
ATACGCC956.0658167E-520.2625166
CGGGAAA24200.019.65872417
TGCTACG1702.8539944E-919.41165419
Produced by FastQC (version 0.11.5)