FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196230
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58690.18362258035247778No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44720.13991483716753803No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT44400.13891365765292235No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT32340.1011817046958448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC34300.045.4777342
GTGGTAT35700.043.345941
GTATCAA127350.040.0253831
TATCAAC146400.034.897182
GGTATCA64550.033.1273731
ATCAACG158200.032.240493
CAACGCA161200.031.6409785
TCAACGC163100.031.3240154
AACGCAG181300.028.417076
CTAGTAC4850.027.1673243
CGCAGAG204450.024.8851768
GTCGACT13300.024.6124211
CGACTTT13300.024.6074143
ACGCAGA210700.024.2071487
CGTCAGA8550.023.8621085
GCAGAGT216600.023.5677579
TCGAGGG48850.023.1446579
TAAGACG26300.021.9791074
AGCGTCA8150.021.9037553
GCGTCAG8700.021.4961344
Produced by FastQC (version 0.11.5)