FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979318
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103551.0573684952181008No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA13070.13346022436021804No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA10480.10701324799503328No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC9940.10149920659070905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTT150.005784079454.99826417
GTGGTAT22500.043.6787871
TGGTATC22600.042.8523862
CGTCTAC1250.037.3988255
ACGTGAA300.001925782836.6655116
CATTGCG1550.033.70861429
TCAGCGC1650.033.33228344
CGTCATT1700.032.3519226
GTCAGCG1800.032.08231743
CCGTGTT701.6089325E-731.4275789
GTCGTCT1550.030.1603453
TCGTCTA1650.029.99905454
GTCGTCA1850.029.72879224
CAGCTCG1850.029.72879237
GTATCAA87950.028.4520071
TGGTCGT1650.028.3324451
CTGTGCG1650.028.332449
GCGCTGG1750.028.28481947
AGCTCGT1850.028.24235238
GCAAGTC400.00787378627.49913433
Produced by FastQC (version 0.11.5)