FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046336
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236042.25587191877179No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18280.174704874915897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT35600.045.4402581
TGGTATC36400.043.9775772
TGCTTAA2450.043.77490219
TGCTACG701.8189894E-1243.21368419
CTGCTTA2900.038.8787718
GCTACGC809.094947E-1237.81197420
ACCACTG4300.037.73203314
CACTGCT3900.037.37127716
CTTAACG458.8442775E-636.66615721
ACTGCTT3150.036.66615717
CAGTGGT2800.035.3718641
CTTAAAC2550.034.50932321
CGGCAAA501.8200133E-532.9995426
TACCACT4850.032.8861413
TTAAACG3050.031.55693822
CGCGCAT350.00409974731.42813532
GATTTCG350.00409974731.42813552
CATGTTA350.00409974731.42813510
GGCATTA350.00409974731.42813520
AGTTACG350.00409974731.42813519
Produced by FastQC (version 0.11.5)