FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555845
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.17714495429356455No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT49980.14055730775666542No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48760.13712633705912378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA4850.051.704573
TGGTATC37550.048.2190132
CGTACCG853.8562575E-1045.0037165
GTATCAA148100.041.4744221
GTGGTAT44800.041.179331
TATCAAC168450.036.813152
GGTATCA71400.035.6612171
CGTCAGA6850.035.367885
GCAGCGT7250.034.0061531
GTACCGT1800.033.058756
ATCAACG193500.032.3329623
CAACGCA193950.032.1049965
TCAACGC197250.031.718274
CAGCGTC8250.029.8808332
AACGCAG210700.029.6136826
GCGTCAG8400.028.841264
TCGACTT13250.027.5867962
CGACTTT13450.027.176583
CGCAGAG235350.026.3299358
GTCGACT14250.025.6537671
Produced by FastQC (version 0.11.5)