FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555845
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147020.4134600917644048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC45650.041.0917552
GTGGTAT51200.037.518641
GCGACGA1500.031.16746923
GTATCAA181300.028.7807541
CGACGAG1700.027.5007124
TATCAAC201600.025.6358282
AGAGTAC101500.024.81663111
ACGAGTC1900.024.60520726
CAGAGTA106350.024.02102910
GGTATCA84400.022.8070433
ATCAACG226250.022.643193
CAACGCA225450.022.5399485
GAGTACG111150.022.34043712
TCAACGC228550.022.3067234
CGTCATC2350.022.23305512
AGTACGG112100.022.00392713
ATCGTCA2250.021.99902210
AACGCAG236750.021.5573436
GTACTAG4500.020.793521
TGCGACG2250.020.77831522
Produced by FastQC (version 0.11.5)