FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694994
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44790.2642487230043292No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT42030.24796547952382134No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38270.22578251014457867No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.2152220007858435No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT31440.18548738225622038No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25400.14985303782786252No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22020.12991196429013907No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA21250.12536917534811334No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21220.12519218357115128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT32400.055.8900261
TGGTATC33250.054.8366972
GTATCAA131050.042.3619461
TATCAAC155800.036.2818872
TCGACTT13050.035.8251342
CGACTTT13300.035.471293
CAGTACG1104.800313E-934.77921
TGTACCG1104.803951E-934.776129
GGTATCA63900.033.7269331
TCGACAT1157.417839E-933.262152
ATCAACG176200.032.2259863
CAACGCA183100.031.0347845
TCAACGC183050.030.903954
GTCGACT16600.028.6800331
TCGAGGG54300.028.6492029
AACGCAG204200.028.0169336
CGTGCAA2053.6379788E-1226.953858
ATACGTG3500.026.7153175
ACATTCG808.608238E-426.5650928
CGCAGAG225700.025.0279188
Produced by FastQC (version 0.11.5)