FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524593
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101871.9418863766767762No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCA150.005781666455.00047724
TAAGCGG150.005781666455.00047748
TCGTGTC257.85223E-444.00037823
CGTCGTG257.85223E-444.00037849
CGCAGCG551.9263098E-839.9965328
TGGTATC11600.039.846922
GTGGTAT12050.037.6954571
TCCGTCT300.00192458836.66698534
TCACCGC401.9011088E-434.372027
ATCAGCG350.004087895631.4438322
GCATCGG350.004097447331.42884446
CCTGACG453.7925664E-430.5558235
GCGTCGA453.794686E-430.55290812
AGTACGT553.489328E-529.997413
GTATCAA48500.029.7427861
ACGCAGC952.4920155E-928.9448597
TGCTTAA1350.028.51876619
ACAAACG606.306852E-527.5028613
GACTTAT400.00786896527.50023830
AGCATCG400.00786896527.50023845
Produced by FastQC (version 0.11.5)