FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012857
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.17060651207426122No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.136544448031657No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.13387872128049666No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA12930.12765869219445589No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12000.11847674449601475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTA250.00779629250.9994962
GTGGTAT10850.048.578141
TGGTATC11800.045.020742
GTATCAA44400.043.367481
TTCCGCG209.3215477E-442.4995876-77
TACGTGC951.1441443E-940.2667356
GGTATCA21100.036.6638341
TATCAAC53250.036.473822
CGACTTT5700.035.0435143
ATCAACG57350.033.940383
TCAACGC59900.032.6374054
CAACGCA60400.032.2968675
GCGTCAG2400.030.103874
TCCGCGT300.006807119628.33305478-79
CCGCGTT300.006807119628.33305478-79
AACGCAG69750.028.2139766
AGCGTCA2500.027.1997323
CGTATAA3600.027.1565321
CTATCCG408.614221E-426.56223920-21
CGCGCGT408.614221E-426.56223930-31
Produced by FastQC (version 0.11.5)