FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012857
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159311.5728775138050088No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA12040.11887166697766811No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGA150.005783269655.00034749
GTGGTAT12600.038.0026971
ACGGGCG300.001926389436.66327716
TGGTATC13900.034.6276472
GTAGCCT350.004099280631.4287729
CGACGGA350.00410126231.42566527
GATGCGG553.4902885E-530.00018945
GTATCAA53350.028.9892921
ACGTGTT400.00787056227.50153221
TTAAACG805.175898E-727.50153222
ACGGGCT507.035139E-427.4974616
CGTAGGT759.7504635E-625.66682843
GATGTCG651.0891571E-425.3835229
AGAGTAC32200.025.02221311
GTATCGG550.001221475325.017451
TATCAAC62250.024.7869592
CAGAGTA32950.024.70303210
CTCGTAG801.6079084E-524.0626541
CGCCGTA801.6079084E-524.0626525
GGTATCA23900.023.6043281
Produced by FastQC (version 0.11.5)