FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780592
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.198182917580503No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11700.14988624018693503No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.148348945415787No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9690.12413655277020516No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.10351118125730215No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT7920.10146145489577142No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGGGG7890.1010771312029844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGC200.003220997363.760486
GTGGTAT8450.051.809931
TGGTATC9000.050.0541532
GCGGTAT452.924097E-547.2269551
GTATCAA38300.045.611621
CGACTTT5650.040.6183363
TATCAAC44450.040.060752
GGTATCA16450.039.532841
TCGACTT6300.035.752972
ATCAACG51300.034.9600373
CAACGCA52300.034.5375755
TCAACGC53400.033.5852054
GTCGACT6850.033.5070081
AACGCAG57200.031.954556
CCTATGC550.004447748430.9082322
CTAGACT1659.094947E-1230.908234
GTGTATA5550.029.8678571
CTGACCC1601.9463187E-1029.2272979
CAGCCGA600.006794553328.3416218
CGGTATC755.904902E-428.3325422
Produced by FastQC (version 0.11.5)