FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124772
Sequences flagged as poor quality0
Sequence length91
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.3502388356362004No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGGGG3860.30936428044753633No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.2933350431186484No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.28692334818709325No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGTGGGGG3190.2556663353957619No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.24765171673131792No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650.21238739460776457No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGTGGCGG2510.20116692847754306No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT2390.1915493860802103No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.17151283941910045No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.14346167409354663No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.1338441316962139No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1550.12422658929888115No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.11941781810021479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.11621197063443722No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.11140319943577084No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTACGCTG1390.11140319943577084No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.10579296637066007No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.10258711890488251No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.10098419517199372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)