FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124772
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82326.597634084570256No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAG150.00577207554.9988026
GAGTACT150.00577207554.99880212
TGAGGTT150.00577207554.9988025
ATATCTG150.00577207554.99880245
CAGTGGT257.799446E-444.0343361
GTGGTAT2900.041.7566951
TGGTATC3000.040.348622
CGGGGTA401.8921158E-434.3742517
ACGGGGT501.807356E-532.9992816
TCGACTT1450.032.25362
AGTGGTA350.00407818831.4404832
GACAGGT350.00408620431.4278839
GTATCAA12950.030.3904911
TCTGGAC506.9974945E-427.499427
CTGGACA506.9974945E-427.499428
GAATTCT506.9974945E-427.499423
CGACTTT1600.027.4993973
AGAGTAC8200.027.1640411
CAGAGTA8300.026.83676510
TATCAAC15200.026.0625082
Produced by FastQC (version 0.11.5)