FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872385
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.3506479364042252No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.2663961439043541No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20870.2392292393839876No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT20470.23464410781936876No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA17120.196243630965686No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.1916584994010672No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT16660.19097072966637435No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC15610.17893475930924993No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT14050.16105274620723647No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13670.1566968712208486No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC12800.14672421006780265No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.1378978318059114No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10570.12116210159505264No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC9790.11222109504404593No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT9490.10878224637058179No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8850.10144603586719166No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG8800.10087289442161432No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC8760.10041438126515242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14100.050.938892
GTGGTAT14950.048.0509641
GTATCAA78100.046.1531521
ACGTGCC701.8960418E-742.5066227
GGTATCA34100.040.3875241
TCGACTT8750.040.313632
TAGCGCA753.2585558E-739.6728489
AGACGTT753.2585558E-739.6728486
TATCAAC95600.038.4983332
CGACTTT9200.038.3417743
TATAGCG805.4041084E-737.1932957
TTCTCTG2750.035.5509957
TATTCTC2750.035.544885
ATTCTCT2850.034.303596
TCTCTGC3050.033.447838
TAGTACG901.3579593E-633.0607071
ATCAACG112650.032.8978233
GACTTTT10850.032.5109984
CAACGCA114400.032.394585
GTCGACT11300.032.350171
Produced by FastQC (version 0.11.5)