FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560295
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94350.20689451011392906No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72750.15952915326749695No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT67740.1485430218878384No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT54050.11852303414581732No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51280.11244886569838136No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT47350.1038310021610444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT51350.045.2823561
TGGTATC54750.043.082942
GTATCAA197800.042.9819221
TATCAAC227850.037.8654752
GGTATCA95900.035.6385151
ATCAACG251000.034.4239043
CAACGCA257200.033.57835
TCAACGC265950.032.457564
AACGCAG274300.031.6578836
AGCGTCA11150.030.1126273
TCGACTT20850.029.5568662
GCGTCAG12250.027.4092394
CGACTTT22350.027.3830263
ACGCAGA317600.027.073257
GTCGACT23850.025.844121
CGCAGAG332950.025.7357318
GCAGAGT334700.025.6900639
TCGAGGG69750.023.8263519
TATACGT5650.023.3194984
CAGAGTA158950.022.7155410-11
Produced by FastQC (version 0.11.5)