FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560295
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237400.5205803571918045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT63550.041.698341
TGGTATC66800.039.0735972
CGACGAG3050.029.7543624
GTATCAA263000.029.463541
TGCGACG3250.028.76948222
TATCAAC299300.025.840582
AGAGTAC148550.025.10177811
CAGAGTA154300.024.30893310
ATCAACG323500.023.6575983
GGTATCA119500.023.5797981
GCGACGA4050.023.08662223
CAACGCA331800.022.981665
CCGCGTA600.002036865822.9158639
TCAACGC334850.022.7479444
AACGCAG343100.022.4491716
TAAGCCG7950.022.13831736
CAATGCG4600.022.11975919
GCCGTCA7850.022.06904839
GAGTACG165600.022.03575912
AGTACGG165050.022.02588713
Produced by FastQC (version 0.11.5)