FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392040
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69780.12941298655054487No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.10081527585106935No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54340.10077818413809986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA173100.040.2250981
GTGGTAT51150.037.979641
TATCAAC196750.035.6206322
TGGTATC55000.035.3141862
CAACGCA216200.032.31845
ATCAACG218200.032.2163623
TCAACGC224700.031.2657974
GGTATCA89600.030.9802881
AACGCAG236600.029.802426
GCGTCAG15200.027.1223834
CGACTTT15100.026.1759053
ACGCAGA274700.025.55997
AGCGTCA16150.024.7372263
GCAGAGT285700.024.471669
CGCAGAG287150.024.2888818
CGTCAGA17300.024.0759665
TCGAGGG58800.024.0699239
GCAGCGT17850.022.6238061
GTCGACT18150.022.2498591
AGAGTAC130500.020.77858410-11
Produced by FastQC (version 0.11.5)