FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392040
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220070.40813866365976514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT58350.037.451381
TGGTATC61350.034.9282952
GTATCAA216850.028.1253911
TATCAAC241600.025.0711862
ATCGGTC1251.7498678E-924.1989314
AGAGTAC124700.024.16916511
ATCAACG264750.022.6658783
CAACGCA263450.022.5564165
CAGAGTA135550.022.3765710
TCAACGC266950.022.2919924
GGTATCA101950.021.6342053
AACGCAG276500.021.5315996
AGTACGG141400.020.9257213
GAGTACG143550.020.6506212
GCGACGA3050.019.83629423
TACGGGG74900.019.49495315
GTACGGG151450.019.02854314
CGCAGAG318600.018.4966878
ACGCAGA321500.018.4068227
ACGGGCG3450.018.33235416
Produced by FastQC (version 0.11.5)