FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878156
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200900.41183594784586636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC50850.035.592982
GTGGTAT52600.035.525171
TCGACGC453.7980825E-430.5545314
GTATCAA179800.027.8875791
ACGCGTT651.0898453E-425.38402411
TATCAAC201400.024.5834312
AGAGTAC102450.022.97652611
ATCAACG215700.022.7324393
TCAACGC217750.022.327614
CAACGCA218700.022.2932625
GGTATCA86250.022.0161861
GCGACGA1658.367351E-1121.66682823
AACGCAG231050.021.2444736
CAGAGTA112550.021.20785710
AGTACGG109750.020.84689313
GAGTACG112500.020.3861912
TCGCGAG700.00497809219.64300223
TACGGGG56100.019.4599315
CGAGGGG21550.018.50304810
GTACGGG123200.018.39205214
Produced by FastQC (version 0.11.5)