FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015289
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT46270.2295948620768535No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.13968219942648424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55900.040.914981
TCGATCG450.001662652537.7809759
GTGGTAT15850.034.867921
TATCAAC65350.034.8596762
GGTATCA27400.034.5992131
GTACTAG2950.033.1449781
ATCAACG68050.033.101823
CAACGCA69850.032.310455
TCAACGC71450.031.4679434
TGGTATC18000.031.1677592
TCGGTCG703.9423275E-430.3597139
CGACTTT5650.029.3373133
AACGCAG76800.029.2772946
GCGTCAG4450.027.6982844
ACGCAGA86150.025.9011447
TATACGT2650.024.0580444
TCGAGGG17150.023.5442669
CTGTGCG4700.023.5126299
GCAGAGT95650.023.2847739
TATCGGT1102.0158313E-423.1832057
Produced by FastQC (version 0.11.5)