FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3863346
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195980.5072804765609914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT45850.038.0574231
TGGTATC49650.034.7886352
GCTAGCG453.7982178E-430.55414638
GTATCAA170700.027.4903531
TATCAAC192550.024.0544552
AGAGTAC91000.024.02330811
CAGAGTA95150.023.14892210
ATCAACG206050.022.1674253
GGTATCA81900.021.8097271
TCAACGC208650.021.7456594
CAACGCA208350.021.7241785
GCGAACG904.0009912E-521.3873539
CGAGCTA650.00324125521.15369236
GAGTACG100600.021.12946712
AACGCAG217700.021.0058786
AGTACGG101500.020.8879313
GTACGGG109500.019.01029214
CGGTTCG1603.0086085E-818.9061118
ACGGGAA31600.018.44803816
CGCGACA750.00741805318.33201440
Produced by FastQC (version 0.11.5)