FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369985
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.26095176224557204No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT33980.2480319127581689No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.2147468767906218No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.20343288430165296No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23860.17416249083019156No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT20850.15219144735161333No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA20260.14788483085581228No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA19370.1413884093621463No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC18780.13708179286634525No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.13591389686748395No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC17430.127227670375953No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15240.11124209389153895No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC15110.11029317839246415No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC14540.10613254889652077No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC200.003222381563.7561576
GTGGTAT17850.055.255531
TACGTCC250.007794718751.0030637
TGGTATC20300.049.61942
GTATCAA92600.043.798921
GGACTAT409.315608E-442.5041056
CTACGGT801.1294105E-842.5010033
TCTAACT409.3189557E-442.5010033
GTCAGAT2350.039.791086
TCCCTGG655.5506553E-639.2431531
GGTATCA44350.039.1104241
CAGTACG1001.888111E-938.2620741
TATCAAC107200.038.179542
GACAGAT1700.037.5118371
CTTAACG602.8740033E-1035.4162120-21
GTAGCGA500.00278835134.0020458
ACGTGTC500.00278835134.0020458
ACCACGT500.002788849934.00085
GTACTAG1404.1836756E-1133.40341
CTGTACC652.5527072E-432.694279
Produced by FastQC (version 0.11.5)