FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369985
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144011.0511793924751No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA14470.10562159439701894No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC13910.1015339584010044No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC13720.10014708190235659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA257.85755E-443.9993910
GTGGTAT24650.041.3165251
CGTTGTG358.645097E-539.3038182
TGGTATC26450.038.3825152
AGGGTCG300.001925883336.66615710
GGCGTAA300.001926228536.66481850
ACTACGG401.9018917E-434.37452313
TCGTAAC501.8204222E-532.99954244
CGTAACC501.8204222E-532.99954245
CCACCGA350.00410018631.42813512
CTGCTTA2400.030.9370718
CTTAACG453.7962344E-430.55512821
GCTTAAC453.7962344E-430.55512820
GTATCAA113050.029.8996961
TGACGGA653.2066364E-629.61497152
GTGACGG653.2075495E-629.6138951
GCTTAAA2100.028.80912220
ACTGCTT2600.028.5562517
TCCCTGG506.994262E-427.525741
GTATTCT705.666612E-627.5257381
Produced by FastQC (version 0.11.5)