FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835293
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58960.20795028944098548No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.14735690455977565No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT41700.14707474677220309No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT39540.13945648650774364No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30830.10873655738577988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29350.046.0551341
TGGTATC31700.042.902322
GTATCAA116650.041.4318921
TATCAAC138000.035.3859672
GGTATCA57750.034.005491
CAACGCA152350.032.1092875
GCAGCGT5100.031.6717781
AACGCAG154950.031.656146
ATCAACG170650.029.0141433
TCAACGC174650.028.276634
ACGCAGA182150.026.7885257
GTCGACT12950.026.5873031
CGACTTT12400.026.39123
GCAGAGT190950.025.6430029
TAGACCG1001.0564359E-425.5000694
TAGCGGT2351.8189894E-1225.3218967
CGCAGAG193300.025.221318
TATATCG1701.19234755E-825.0005075
CGTCAGA7150.024.9655445
TCGACTT13750.024.418252
Produced by FastQC (version 0.11.5)