FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309907
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.18287522882062848No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45560.1376473719654359No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT43090.13018492664597525No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.10081854263579008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGT1150.044.35368
GTATCAA132100.041.9926031
TGGTATC35050.041.957912
GTGGTAT35000.041.6573071
AGCGTCA6150.037.3202363
GGTATCA67150.035.1327441
TATCAAC162200.034.6684722
ATCAACG169850.033.207113
CAACGCA169450.033.1375055
TCAACGC176900.031.811634
GCGTCAG8000.029.2212264
AACGCAG197750.028.848346
CGTCAGA8400.027.8318425
CGACTTT12400.027.7644523
CGCAGAG206700.027.1648798
GCAGAGT217450.025.9392229
TCGAGGG46750.025.3669369
ACGCAGA223150.025.3147417
GTCGACT13800.025.2580531
TCGACTT13950.024.6795122
Produced by FastQC (version 0.11.5)