FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4581932
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76480.16691648850310306No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA63490.13856600228899077No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58670.12804642233887364No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT54060.1179851643367907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT44200.044.4367681
GTATCAA169200.043.3673741
TGGTATC48650.040.0129972
TATCAAC196550.037.8211942
GGTATCA82250.034.4756621
CAACGCA217550.034.0147745
ATCAACG221750.033.446473
AGCGTCA9550.032.044063
TCAACGC236850.031.385924
GCGTCAG10450.029.2842854
AACGCAG260200.028.8989226
CGCAGAG261700.028.0973638
GCAGAGT270300.027.234859
TCGACTT15500.025.7759462
ACGCAGA289500.025.7075527
CGACTTT16200.025.1868953
CGTCAGA13250.024.3795455
TCGAGGG64200.022.3771539
CAGAGTA137250.021.69296610-11
CTGTGCG8500.021.5016969
Produced by FastQC (version 0.11.5)